Journal: The Journal of Biological Chemistry

Article Title: Solution Structures of Complement C2 and Its C4 Complexes Propose Pathway-specific Mechanisms for Control and Activation of the Complement Proconvertases *

doi: 10.1074/jbc.M116.722017

Figure Lengend Snippet: Half-lives ( t ½) in seconds for dissociation of C4b2 and C4b2a formed on the surface of an SPR sensor chip The values were obtained by analysis of two independent experiments by the one-phase exponential decay model in GraphPad. R 2 goodness of each fit of a model to the data was >0.99.

Article Snippet: The correlation between RALS and RI (the red line of log of molecular mass estimation) suggests that the eluate is close to being monodisperse. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 C4b2 at 25 °C ( D, curves ) C4b2a at 25 °C ( E, curves ) C4b2a at 15 °C C2 WT, Mg 2+ buffer 291, 246 ( black ) 1457, 1483 ( black ) 4124, 4729 C2 sm, Mg 2+ buffer 498, 420 ( red ) 2667, 2689 ( red ) 5954, 7748 C2 WT, Ni 2+ buffer 480, 486 ( green ) 2527, 2303 ( green ) C2 sm, Ni 2+ buffer 665, 684 ( blue ) 4387, 4305 ( blue ) Open in a separate window Half-lives ( t 1⁄2) in seconds for dissociation of C4b2 and C4b2a formed on the surface of an SPR sensor chip The values were obtained by analysis of two independent experiments by the one-phase exponential decay model in GraphPad.

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